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4.3. Construction and Transformation of Binary Vectors

YZ Yanjun Zhang
SH Shaqila Han
YL Yuqing Lin
JQ Jiyue Qiao
NH Naren Han
YL Yanyan Li
YF Yaning Feng
DL Dongming Li
YQ Yanhua Qi
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The open reading frame of OsPIN1b was amplified from the cDNA of WT/NIP, and the 2.6 kb promoter of the OsPIN1b was amplified from WT/NIP genomic DNA. 35S:OsPIN1b-sGFP and ProOsPIN1b:GUS vectors were constructed as described previously [25]. These vectors were introduced into Agrobacterium strain EHA105 and transformed into WT/NIP calli. Primer sequences are listed in Table S1.

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