4.10. RNA-Seq Analysis

Total RNA of each sample was extracted with TRlzol Reagent (Life technologies, Carlsbad, CA, USA). The purity, concentration, and integrity of the total RNA samples were assessed before further analysis. The RNA-seq libraries were constructed using Illumina TruSeq RNA sample kit (Illumina, San Diego, CA, USA). DESeq2 performed gene-expression analysis of the different groups. Genes with a p-value of <0.05 were defined as differentially expressed genes (DEGs).