Cells were seeded in 6-well plates and treated with the corresponding drug for 24–72 h. After removing the 6-well plate, discard the old full medium, wash with PBS twice, carefully drain the remaining liquid with a pipette, fix the cells with methanol for about 15–20 min at room temperature and then dump them, and carefully wash with PBS twice. Observe the morphology and number of cells under the white light lens of the microscope and take pictures.
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