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ChIP (chromatin immunoprecipitation) assay

SG Shuyu Guo
YZ Yuxin Zhang
TZ Tingting Zhou
DW Dongyue Wang
YW Yajuan Weng
LW Lin Wang
JM Junqing Ma
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ChIP assays were performed using EZ-ChIP (Millipore Corporation) according to the manufacturer’s protocol40. Briefly, SCAPs were cross-linked with 1% formaldehyde at 37 °C for 10 min; subsequently, the cells were lysed in SDS buffer to shear DNA by sonicate. Lysates diluted with ChIP dilution buffer were immunoprecipitated with anti-GATA4 (ab86371, Abcam) or rabbit IgG as an internal control. PCRs were performed to amplify the fragment of GNAI3 promoter using 2 μL of the extracted DNA (with or without antibody) as a template. PCR products were electrophoresed on 2% agarose gel stained with ethidium bromide and visualized under UV light.

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