Western blot analysis

Briefly, B16/F10 cells or tumor tissues were lysed with RIPA buffer (Beyotime Biotechnology, catalog no. P0013B) containing protease inhibitor cocktail (Thermo Fisher Scientific, catalog no. 78425). Nuclear proteins were obtained using a Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Biotechnology, catalog no. P0027). The protein concentration was evaluated by the BCA Assay Kit (Beyotime Biotechnology, catalog no. P0010S), and 30 μg of total protein were electrophoresed via SDS-PAGE and blotted onto a polyvinylidene fluoride (PDVF, Beyotime Biotechnology, catalog no. FFP33) membrane. After blocking, the PVDF membranes were incubated with various primary antibodies including anti-YTHDF1 (1:2000; Abcam, catalog no. ab220162), anti-YTHDF2 (1:1000; Abcam, catalog no. ab220163), and anti-YTHDF3 (1:1000; Abcam, catalog no. ab220161) at 4 °C overnight. On the next day, the PVDF membrane was incubated with the second antibody (1:5000) for 2 h and washed three times with TBST. The bands were then imaged using a Bio-Rad system following the instructions. The uncropped blots for the main and supplementary figures are provided in the ^{Source Data} file.