Statistical analysis

All experiments were repeated at least three times. All statistical analysis was performed with GraphPad Prism Version 8.3 (GraphPad 8.3 Software, San Diego, CA, USA). The differences of survival rates in treated versus EV-A71 mice were assessed with the Mantel-Cox log rank test and survival curves were plotted using the Kaplan-Meier method. The results were expressed as the mean ± standard deviation (SD) or median with range (non-normal distribution). Statistical analysis was carried out using two-tailed unpaired Student’s t test or Mann-Whiteny test (non-normal distribution) for two groups. Differences between groups were considered as significance when the P value was less than 0.05.