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Phenotypic characterization and the relative water content

YZ Yue Zhang
RZ Ruihai Zhang
ZS Zhen Song
WF Weidong Fu
LY Lingling Yun
JG Jinhui Gao
GH Guang Hu
ZW Zhonghui Wang
HW Hanwen Wu
GZ Guoliang Zhang
JW Jiahe Wu
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The plant height, root length, and root/shoot ratio were measured respectively. The relative water content (RWC) of aerial parts of I. lactea var. chinensis was measured using the method of Parida et al. [76]. The dry weight resulting from each treatment was obtained by drying at 80 °C for 8 h. RWC was calculated using the following formula: RWC (%) = (FW-DW)/FW × 100 (FW: fresh weight and, DW: dry weight).

Three treatment’s fresh leaves proline concentration and ROS accumulation were quantization respectively. Proline concentration was determined with acid ninhydrin reagent followed by Bates et al. [77] methods and formula: μmoles proline/g of fresh weight material = [(μg proline/ml × ml toluene) / 115.5 μg/μmole]/[(g sample)/5]. The accumulation of H2O2 and O2·- production was quantization the ROS activity. H2O2 and O2·- was histochemical detection with diaminobenzidine (DAB) and nitroblue tetrazolium (NBT), respectively followed by Romero-Puertas et al. [78].

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