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H9c2 cells were inoculated in 96-well plates with 10 μL CCK8 reagent per well (Dojindo, Kumamoto, Japan). After 2h, microplate readers were employed to measure each well's absorbance (OD) at 450 nm in the dark. Blank wells contained only medium without cells, whereas control wells contained cells without treatment. Cell viability = (OD sample - OD blank)/(OD control - OD blank).
Copyright and License information: ©2024 HCFMUSP. Published by Elsevier España, S.L.U.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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