2.5. Analysis of C. elegans intestinal pathology

The wild-type C. elegans N2 and the transgenic C. elegans FT63 (DLG::GFP) were fed with Rho-labeled MCP03 and unlabeled MCP03, respectively. Bovine serum albumin (BSA) was used as the negative control. After 72 h of incubation, the worms were rendered unconscious using a 15 mmol L⁻¹ sodium azide and fixed on a microscopic slide coated with 2 % agarose for imaging. The worms were examined using a fluorescence microscope (80i, Nikon, Japan) in two different modes: green fluorescence (GFP) and red fluorescence (RFP).