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In vivo assessment of the therapeutic potential of N-Ad-MSCs and DM-Ad-MSCs in STZ-induced diabetic rats

OB Omar I. Badr
MK Mohamed M. Kamal
SE Shohda A. El-Maraghy
HG Heba R. Ghaiad
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Induction of DM by STZ injection in rats

T1DM in 24 female Sprague–Dawley healthy rats was induced by subcutaneously injecting STZ (50 mg/kg in Citrate buffer). The same inclusion and exclusion criteria described earlier in this study were applied. On the other side, 6 normal non-diabetic rats were selected to be as a control group. FBG and body weight were measured after 72 h of STZ induction. 10 days after induction of diabetes; 18 STZ rats were randomized using simple randomization method into the 3 groups; and then they were categorized into three groups; STZ + PBS group (6 non-treated STZ-induced diabetic rats), STZ + DM-Ad-MSCs group (6 STZ-induced diabetic rats treated with DM-Ad-MSCs in PBS), STZ + Normal-Ad-MSCs group: (6 STZ-induced diabetic rats treated with N-Ad-MSCs in PBS).The animals groups were blindly checked for FBG and BWs by an independent technician.

Transplantation of Ad-MSCs

On the day of injection, each fasted rat from STZ + DM-MSCs and STZ + Normal–MSCs group received 1–1.5 × 106 freshly trypsinized P3-P4 (DM-Ad-MSCs) and (N-Ad-MSCs), respectively, where cells were suspended in 1 mL PBS. Cells suspended in PBS were transplanted into tail veins using a 1 mL 100-unit 27-gauge insulin syringe. FBG and BW were measured every 7 days after the day of transplantation for 35 days only. Transplantation was done by the same experimenter for all animals and blindly regarding the groups.

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