Total RNA was extracted by TRIzol reagent (15596026), which was obtained from Thermo Fisher Scientific. The mRNA reverse transcription kit (CW2569) was purchased from CoWin BioSciences. We searched target gene sequences in the NCBI database and designed the primers with Primer5 software. The primers were synthesized by Sangon Biotech. Sequences of the qPCR primers used in this study are outlined in Table 2.
Sequences of the qPCR primers.
H: human; M: mouse; F: forward; R: reverse
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