4.6. Gel Electrophoresis

Amplified PCR products were resolved by gel electrophoresis on 2% agarose gels (#IB70051, IBI Scientific, Dubuque, IA, USA). Subsequently, the gels were stained with ethidium bromide (#E1510, Sigma-Aldrich, Burlington, MA, USA) for 30 min. The DNA bands were visualized using ChemiDoc™ MP (Bio-Rad, Carlsbad, CA, USA), and their sizes were estimated by comparison to a 100 bp ladder (#SM0243, Thermo Scientific, Waltham, MA, USA).