4.3. Sol–Gel Transition Behaviors of TA/PluF Hydrogels

To obtain the sol–gel transition curves of TA/PluF hydrogels, a vial-inverting test with temperature changes between 4 and 100 °C was performed using a refrigerated/heating circulator (JEIO Tech, Daejeon, Republic of Korea) equipped with a water bath. Briefly, TA/PluF solutions (500 μL) with various concentrations were placed in 2 mL test tubes, which were then placed in the water bath of the refrigerated/heating circulator at 4 °C and stabilized for 5 min. The temperature of the water bath was gradually increased at a rate of 1 °C per 5 min. The sol–gel phase transition temperature of the TA/PluF hydrogels was monitored when no flow was observed for 5 min after inverting the test tubes. PluF hydrogels with and without TA were used as controls. All samples were analyzed in triplicate.