Quantitative real-time polymerase chain reaction (q-PCR)

XD Xiangyang Deng
JR Junwei Ren
KC Kezhu Chen
JZ Jin Zhang
QZ Quan Zhang
JZ Jun Zeng
TL Tianwen Li
QT Qisheng Tang
JL Jian Lin
JZ Jianhong Zhu
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The surrounding brain tissues of the hematoma were used for q-PCR analysis. Total RNA was extracted using the RNA extraction reagent (TRIzol, Invitrogen) following the manufacturer's instructions. Subsequently, cDNA synthesis and subsequent qPCR were performed using the Servicebio® RT First Strand cDNA Synthesis Kit (Servicebio) and SYBR Green qPCR Master Mix (without ROX, Servicebio). The synthetic primer sequences are listed in Additional file 1: Part S2.

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