Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
All oligos, sgRNA, and primers used in this study are listed in Supplementary Table 1 . The pLV3-CMV-KHDRBS1-EGFP-Puro plasmid and its corresponding control, pLV3-CMV-EGFP-Puro, were procured from Miaoling Biology (Wuhan, China). KHDRBS1 sgRNA was custom-designed, synthesized, and then ligated into the lentiCRISPR-EGFP vector. This construct was employed to generate the lentiCRISPR-EGFP sgRNA construct, which was subsequently verified by DNA sequencing. Transfections were carried out using.
Copyright and License information: ©2024 Fan, Liu, Gong, Liu, Tang and Shen
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
