Isolation of PBMCs and generation of EBV-transformed cell lines

PBMCs were separated from heparinized peripheral blood samples obtained from a healthy control and the patient by density gradient centrifugation using Histo Paque 1077 (Sigma-Aldrich). EBV-transformed cell lines (EBV-LCLs) were generated by in vitro transformation of human B cells with EBV (Stain B95-8) as described previously (Yamada et al., 2012). EBV-B cells were grown in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin. To generate monocyte-derived macrophages (MDMs), CD14-positive cells were sorted using CD14 microbeads (Miltenyi Biotec), suspended in RPMI supplemented with 10% FBS, penicillin/streptomycin, and 5 ng/ml G-CSF, plated (1 × 10⁵ cells per well) into 96-well plates, and incubated for 7 days.