Virus-induced gene silencing

Functional analyses of CsPrx73 and CsERF7-3 were conducted using the VIGS system based on the cucumber green mottle mosaic virus, following the method of Liu et al. [82]. The CsPrx73 (from +91 to +390 bp) and CsERF7-3 (from +211 to +510 bp) coding sequences were amplified from 9930 (Supplementary Data Table S10). The sequences were then inserted into the BamHI site of pV190 vectors to generate pV190-CsPrx73 and pV190-CsERF7-3, respectively. Following the verification of the accuracy of the sequence constructs through Sanger sequencing, the recombinant constructs were introduced into Agrobacterium tumefaciens strain GV3101 cells. The Agrobacterium-mediated transformation and infection followed the methods described previously [82]. Primers used for VIGS assay are listed in Supplementary Data Table S10.