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Plasmids and GST-tagged protein expression
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The pGEX-6P1 plasmids containing the full length HuR were generated as previously described (70). The GST-HuRG224D plasmid was generated by Norclone Biotech Laboratories. BL21 bacteria were transformed with either GST or the GST-HuR constructs described above. The expression of the proteins was induced by IPTG (0.5 mM for 4 h at 37°C) in a 1-l culture. The bacteria were collected and lysed. The GST proteins were pulled down using Glutathione Sepharose beads.
Copyright and License information: ©2024 The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.
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