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Sampling of V. mirabilis colonies

BT Bastien Taormina
TK Tina Kutti
SO Siri Aaserud Olsen
PS Pål Næverlid Sævik
RH Rita Hannisdal
VH Vivian Husa
EL Erwann Legrand
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Sea pens were sampled at different distance from the farm to measure the concentration of EMB in the tissue. Three colonies were sampled at sites A1, A2, A3 and A4 (respectively 2110 m, 1200 m, 940 m, and 680 m away from the farm) on 03/02/2023 and five colonies were sampled at site C1 (directly below the farm) on 03/03/2023, using the manipulator arm of the ROV. Exact coordinates of each seas pen are available in Supplementary Fig. S3. Calcium carbonate skeleton the sea pens was carefully removed, using disposable gloves, and carefully cleaned dissecting equipment, remaining tissue has been transferred into 50 mL Falcon tube and stored in − 20 °C freezer pending emamectin analyses described in part 5.6.

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