In order to explore the effect of TFs regulating gene expression on the occurrence and development of CIS, the regulation network of TF-hub gene was built based on TRRUST (https://www.grnpedia.org/trrust/) database36. In addition, real hub gene related drugs were identified from the DGIdb (https://dgidb.org/) database37, followed by molecular docking by AutoDock Vina 1.1.2 software38. The docking results were visualized through PyMol 2.539.
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