Transfection and plasmid constructions

All transfection experiments were meticulously conducted using the highly effective Effectene Transfection Reagent (Qiagen GmbH), strictly following its meticulously detailed recommendations. The essential reagents, including DYRK1A siRNA (si-DYRK1A), miR-298 mimic, miR-298 inhibitor, and the negative control (NC), were all procured from the esteemed supplier Gene Pharma (Shanghai, China). Moreover, for the purpose of this study, the cDNA corresponding to human DYRK1A was skillfully amplified through PCR and subsequently integrated into the pcDNA3.0 empty vector (Thermo Fisher Scientific) to meticulously engineer the pcDNA-DYRK1A vector.