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Treponeme specific PCR

JW Jennifer H. Wilson-Welder
SH Sushan Han
DB Darrell O. Bayles
DA David P. Alt
CK Carly Kanipe
KG Kyle Garrison
KM Kristin G. Mansfield
SO Steven C. Olsen
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PCR for Treponema medium, Treponema pedis, Treponema phagedenis, Treponema vincentii, Treponema denticola, Treponema brennaborense and Fusobacterium necrophorum was performed on n = 158, including healthy, lesion and contralateral samples, in a two-stage nested PCR assay as previously described [1, 17, 18]. Positive controls were genomic DNA purified from each respective bacterial species. Details on bacterial strains, PCR primers and amplification conditions are given in supplementary material 5 (Table S1). Sterile water was run in each PCR assay as a negative control. PCR reaction products were visualized by electrophoresis on 1.5% agarose gel stained with SYBR Safe DNA gel stain (Invitrogen Life Technologies, Carlsbad CA, USA).

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