Subcellular localization analysis of GhDMT6

XY Xiaomin Yang
ZB Zhigang Bai
YH Yunxin He
NW Ning Wang
LS Liangqing Sun
YL Yongqi Li
ZY Zujun Yin
XW Xiaoge Wang
BZ Binglei Zhang
MH Mingge Han
XL Xuke Lu
XC Xiugui Chen
DW Delong Wang
JW Junjuan Wang
SW Shuai Wang
LG Lixue Guo
CC Chao Chen
KF Keyun Feng
WY Wuwei Ye
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GhDMT6 gene was ligated into pBI121 vector containing red fluorescent protein to construct GhDMT6-RFP subcellular localization vector. The positive control, no-load control and GhDMT6-RFP vector were transferred into agrobacterium tumefaciens (LBA4404). Tobacco leaves were transfected with agrobacterium tumefaciens at 6 weeks after incubation in an indoor growth chamber, and instantaneously transformed tobacco leaves were used to visualize and locate RFP proteins under confocal laser scanning microscopy [75].

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