GhDMT6 gene was ligated into pBI121 vector containing red fluorescent protein to construct GhDMT6-RFP subcellular localization vector. The positive control, no-load control and GhDMT6-RFP vector were transferred into agrobacterium tumefaciens (LBA4404). Tobacco leaves were transfected with agrobacterium tumefaciens at 6 weeks after incubation in an indoor growth chamber, and instantaneously transformed tobacco leaves were used to visualize and locate RFP proteins under confocal laser scanning microscopy [75].
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