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A wound healing assay was performed to detect the migratory ability of CRC cells. First, CRC cells were seeded in 6-well culture plates at 5000 cells/well. Then, the CRC cells were incubated with OXA at 6 μg/mL for 24 h. A sterile pipette tip was used to create a wound. The cells were then imaged at 0 and 24 h after wounding. Experiments were performed in triplicate.
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