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Protein determination

SB Suhas Bobba
KC Kuldeep S. Chauhan
SA Sadia Akter
SD Shibali Das
EM Ekansh Mittal
BM Barun Mathema
JP Jennifer A. Philips
SK Shabaana A. Khader
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BMDMs were either stimulated with Mtb antigens for 1 day or left unstimulated, lysed in RIPA Lysis Buffer (Santa Cruz Biotechnology), and heat denatured in reducing sample buffer (Thermo Fisher Scientific). Proteins were separated in 4% to 20% polyacrylamide gradient gels (Bio-Rad) and transferred onto PVDF membranes. Membranes were probed with primary antibodies against IFNAR1 (R&D, Cat#AF3039), and GAPDH (Santa Cruz Biotechnology, Cat# SC-25778) followed by incubation with HRP-conjugated anti–rabbit IgG secondary antibody (Santa Cruz Biotechnology) and developed by Clarity Western ECL Substrate (Bio-Rad).

Copyright and License information:  ©2024 Bobba et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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