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Total RNA was extracted from fresh mouse kidneys of each group using the TRIzol method, with samples having no DNAse/RNAse activity. Then, a 1 µg sample of RNA was reverse-transcribed into cDNA using the GoScript Reverse Transcription System, according to the manufacturer’s protocol (Promega). The qRT-PCR was performed on an ABI7500 system using the GoTaqR qPCR Master Mix (Promega). All data were normalized to the expression of β-actin, and relative RNA expression was calculated using the 2−δδCT method. All primer sequences are in Supplementary Table 1.
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