Construction of a rat VSMC osteogenic induction model

Wei Lin; Lianglei Hou; Jialyu Tang; Anwu Huang; Zhuyin Jia

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Construction of a rat VSMC osteogenic induction model

WL Wei Lin

LH Lianglei Hou

JT Jialyu Tang

AH Anwu Huang

ZJ Zhuyin Jia

This method is extracted from research article: BMC Cardiovasc Disord, Apr 2024

Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

DOI: 10.1186/s12872-024-03891-2

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Model group rat VSMCs were cultured in an incubator at 37 °C in a 5% CO₂ atmosphere. On reaching 80–90% confluence, the cells were digested with 0.25% trypsin and used to inoculate the wells of six-well plates containing 2 mL of complete medium at a cell density of 1 × 10⁵ cells/well. The cells were incubated for 24 h at 37 °C in a 5% CO₂ atmosphere, after which, the plates were removed and the complete medium was carefully aspirated from the wells, followed by the addition of 2 mL of osteogenic induction medium containing 10 mM β-GP (D302990; Aladdin, China). The cells were thereafter induced for 0, 3, 7, and 14 d for subsequent assays. Control cells were cultured normally in the absence of osteogenic induction.

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