2.3.1. Preparation of the inoculum of myxobacteria and pathogenic bacteria

The strain WCH05 was activated and inoculated into 3 mL of LBS liquid medium at 30°C and 160 rpm for 2 days. It was then inoculated into 200 mL of VY/2 medium at 30°C and 160 rpm for 3 days. The bacterial pellet at the bottom of the bottle was fully dispersed with a pipette to obtain the myxobacteria inoculum.

The E. amylovora was activated by picking a single colony into LB liquid medium. It was then cultured in a shaker at 28°C and 160 rpm for 24 h until the OD₆₀₀ of the culture reached 1.0. It was then diluted with sterile water to a concentration of 10⁷ cfu·mL⁻¹ as the inoculum.