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To assess mitochondrial mass and shape, polg2+/ia304 adults, bearing the mitoGFP Tg(Hsa.Cox8a:MLSEGFP)ia301 transgene, were inter-crossed. The fluorescent offspring was mounted in 1% agar (in fish water) and imaged using a Nikon Eclipse 90i microscope in a C2 confocal system. Signal quantification was performed using ImageJ software and results were normalized twice, using area and average integrated density, in comparison with wt controls, as described in Facchinello et al. [26].
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