2.4.4. Angiogenesis-related gene levels

After 3 days in culture, HUVECs were harvested and reverse transcribed to cDNA and RT-PCR was performed to check the gene expression levels of Willebrand Factor (vWF), vascular endothelial growth factor (VEGF), CD31, and β-actin as a control reference. Sequence information used in the study is presented in Table 1.

Primer sequences of each angiogenesis-related gene