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2.5. Drosophila sample preparation
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Drosophila egg chambers were prepared as previously described with minor modifications [24]. Briefly, flies expressing the microtubule-associated protein, Jupiter, endogenously tagged with eGFP were fattened on dry yeast at 25°C for one day before dissection [25]. Their ovaries were dissected at room temperature in a drop of Voltalef oil 10S (VWR, 24627.188) on a cover glass (22 × 50, thickness No. 1.5, VWR, 631-0138) and individual egg chambers were isolated and positioned at the centre of the coverslip.
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https://creativecommons.org/licenses/by/4.0/2334-2536/23/070880-08Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
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