4.2. Scientific Equipment

The investigated rhizome of Alpinia officinarum and all reagents were weighed on AXIS AGN200 (Gdansk, Poland) and Radwag WPS 110/C/1 (Radom, Poland) scales. During the extraction process, an INFORS AG shaker (Bottmingen, Switzerland) and the EMAG EMMI 55 ultrasonic bath (Juszczyn, Poland) were used. The following were also used: to filter the extracts—the SBS-LZ-4008 electric centrifuge, Steinberg Systems (Berlin, Germany) and tissue paper filters type 388 (Munktell, Falun, Sweden), and to concentrate them—the INGOS RVO400 vacuum evaporator (Prague, Czech Republic) and Eppendorf Concentrator Plus (Warsaw, Poland). The prepared extracts were mixed with the solvent (methanol) using a VELP Vortex ZX4 shaker with an infrared sensor (Usmate, Italy). Before chromatographic analysis, all samples were filtered through a syringe filter (CronusFilters, Ø pores 0.22 µm). For chromatographic studies, the following devices were used: in the qualitative analysis—the HPLC-ESI-QTOF-MS/MS (High Performance Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight-Mass Spectrometry) platform (Agilent Technologies, Santa Clara, CA, USA) with the Zorbax Eclipse Plus RP-18 column (d_p = 3.5 µm; 2.1 mm × 150 mm), in the quantitative analysis—LC-2030C 3D Plus liquid chromatograph coupled with a UV-Vis-DAD detector (Shimadzu, Japan) and a Zorbax Eclipse Plus RP-C18 column (d_p = 3.5 µm; 2.1 mm × 150 mm) (Agilent Technologies, Santa Clara, CA, USA), and for spectrophotometric tests—UV-Vis EVO300 PC spectrophotometer, Thermo Fisher (Waltham, MA, USA). The system for qualitative determinations of the extract also included: a degasser (G1322A), an autosampler (G1329B), a binary pump (G1312C), a photodiode detector—DAD (G1315D) and a mass spectrometer (G6530B). To acquire the MS spectra and process the obtained data, Mass Hunter Workstation software (Agilent Technologies, Santa Clara, CA, USA, B.10.00 version) was used.