Measurement of plasma parameters and blood glycated hemoglobin levels

KT Kazumi Take
TM Taisuke Mochida
TM Toshiyuki Maki
YS Yoshinori Satomi
MH Megumi Hirayama
MN Masanori Nakakariya
NA Nobuyuki Amano
RA Ryutaro Adachi
KS Kenjiro Sato
TK Tomoyuki Kitazaki
ST Shiro Takekawa
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Blood samples were collected from the tail vein without anesthesia. Plasma glucose, TG, cholesterol and non-esterified fatty acid (NEFA) levels were measured with a 7180 autoanalyser (Hitachi High-Technologies Co., Tokyo, Japan). Blood glycated hemoglobin (GHb) levels were measured with automated GHb analysers (HLC-723G8, Tosoh, Tokyo, Japan). Plasma insulin levels were measured using a mouse insulin ELISA kit (Shibayagi, Gunma, Japan).

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