Blood samples were collected from the tail vein without anesthesia. Plasma glucose, TG, cholesterol and non-esterified fatty acid (NEFA) levels were measured with a 7180 autoanalyser (Hitachi High-Technologies Co., Tokyo, Japan). Blood glycated hemoglobin (GHb) levels were measured with automated GHb analysers (HLC-723G8, Tosoh, Tokyo, Japan). Plasma insulin levels were measured using a mouse insulin ELISA kit (Shibayagi, Gunma, Japan).
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