Materials

Structure of compA was shown in Fig 1A. Synthesis of compA was reported previously [20]. This compound exhibited selectivity (greater than 30,000-fold) against related acyltransferases (MGAT3, DGAT1, DGAT2, and ACAT1) [20]. Glycerol-labeled MG (2-oleyl-[1, 1, 2, 3, 3 d₅]-glycerol) and fatty acid-labeled MG (2-[17, 17, 18, 18, 18 d₅]-oleoylglycerol) were purchased from CURACHEM (Gyeonggi-do, Korea). Organic solvents were purchased from WAKO (Osaka, Japan).

Fasted C57BL/6J mice were given a liquid meal orally with intraperitoneal injection of Pluronic F-127 to inhibit plasma TG lipolysis. (A) Structure of compA. Plasma samples were collected at 0, 2, and 4 h after oral gavage of a liquid meal. (B) Time course of changes in plasma chylomicron TG (CM/TG) levels and (C) postprandial TG excursion of 3 or 10 mg/kg compA at 6 h after dosing. (D) Time course of changes in plasma TG levels and (E) postprandial TG excursion of 30 mg/kg compA at 16 h after dosing. n = 6 (B, C), and n = 7 (D, E). #: P < 0.025 vs. vehicle group by one-tailed Williams’ test. **: P < 0.01, ***: P < 0.001 vs. vehicle group by Student’s t-test.