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2.16 Immunohistochemistry
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Lung sections (3 μm) were incubated with primary antibodies against p53 (1:100), Cleaved-PARP (1:100), Bax (1:100), and Cleaved-caspase 3 (1:100). On the second day, the slices were exposed to the corresponding secondary antibody for 1 hour and observed under a microscope.
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