4.3. Isolation of Exosomes

From CAP-Lamp2b or hygro-Lamp2b plasmid DNA-transfected BM-MSCs, cell culture-conditioned media exosomes were isolated and purified either by differential centrifugation as described by Thery et al. in [18] or by using the ExoEasy Maxi Kit (Qiagen, Venlo, The Netherlands). Final ultracentrifugation was carried out at 100,000× g using Beckman Model L8-70MR ultracentrifuge.