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High pressure liquid chromatography analysis

DP Diptikanta Padhan
SP Smaranika Pattnaik
AB Ajaya Kumar Behera
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The HPLC analysis of Spilanthes flower EO was performed at Sophisticated Analytical Instrument Facility (SAIF, DST), CSIR-Central Drug Research Institute (CDRI), Lucknow, India, using Waters HPLC Model-515 with PDA detector. The chromatographic column used for the analysis was Inertsil CN-3, (250 mm × 4.6 mm, 5 μm as particle size). An isocratic separation was conducted with mobile phase composition of n-hexane and isopropanol (1:15). The flow rate was 1.0 ml/min. The process was monitored at wavelength (λ) 250 nm.

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