2.15. Cytokine Assay

HJ Hua Jin
YZ Yue Zhao
YY Yinlian Yao
SF Shilong Fan
RL Renxing Luo
XS Xin Shen
YW Yanyan Wang
JP Jiang Pi
GH Gonghua Huang
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The whole blood with EDTA originating from septic mice was centrifuged at 3000 rpm for 10 min at 4 °C, and the plasma was isolated for ELISA assay of IL-6 and IL-1β levels according to the protocols provided by the manufacturer (Thermo Fisher Scientific, Waltham, MA, USA).

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