All housing, breeding, and procedures/experiments were performed according to the NIH Guide for the Care and Use of Experimental Animals and approved by the Van Andel Institute Institutional Animal Care and Use Committee (IACUC). Mus musculus C57BL/6J mice were purchased from the Jackson Laboratory (000664; RRID:IMSR_JAX:000664) and housed at 22 °C and 50% humidity with 12-hour light cycles (lights on at 7 am, lights off at 7 pm). Both male and female mice were used and were 3-4 months old at the time of injection. Animals were aged 6–7 months old prior to sample collection. 5 male and 6 female mice were utilized in the GeoMx experiments and 1 female and 2 male mice were used for CosMx.
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