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Transmission electron microscopy

HD Higinio Dopeso
PR Paulo Rodrigues
FC Fernando Cartón-García
IM Irati Macaya
JB Josipa Bilic
EA Estefanía Anguita
LJ Li Jing
BB Bruno Brotons
NV Núria Vivancos
LB Laia Beà
MS Manuel Sánchez-Martín
SL Stefania Landolfi
JH Javier Hernandez-Losa
SC Santiago Ramon y Cajal
RN Rocío Nieto
MV María Vicario
RF Ricard Farre
SJ Simo Schwartz, Jr.
SI Sven C.D. van Ijzendoorn
KK Kazuto Kobayashi
ÁM Águeda Martinez-Barriocanal
DA Diego Arango
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Jejunum samples were collected from 90-day-old Vil-Cre+;RhoAT19N (n = 3) and control Vil-Cre–;RhoAT19N mice (n = 3). Samples were fixed with 2.5% glutaraldehyde and 2% paraformaldehyde and processed following standard procedures. Ultra-thin sections were mounted on copper grids, contrasted with uranyle acetate/lead citrate double-staining, and observed in a Jeol JEM-1400 (Jeol LTD) transmission electron microscope equipped with a Gatan Ultrascan ES1000 CCD camera. The brush border architecture was evaluated on at least 12 enterocytes per mouse. Microvillus length and density (microvilli/μm) were measured using ImageJ software as was reported previously.71

Copyright and License information:  ©2024 The Authors
This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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