The HPLC analysis was performed using a Shimadzu LC-20AT equipped with an SPD-20A UV-detector (Yu et al., 2023). An Agilent ZORBAX SB-C18 (250 × 4.6 mm) column was employed. The parameters of Shimadzu HPLC system were listed as follows: wavelength: 270 nm (VAN, PRO and SIN) and 220 nm (STI), oven temperature: 30 °C, solvent: methanol with 0.1% formic acid, flow: 1.0 mL/min, injection: 10 μL.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.