4.6. Hydroxyproline Assay

PO Phoebe Ohene-Marfo
HN Hoang Van M. Nguyen
SM Sabira Mohammed
NT Nidheesh Thadathil
AT Albert Tran
EN Evan H. Nicklas
DW Dawei Wang
RS Ramasamy Selvarani
JF Jacob W. Farriester
RV Rohan Varshney
MK Michael Kinter
AR Arlan Richardson
MR Michael C. Rudolph
SD Sathyaseelan S. Deepa
request Request a Protocol
ask Ask a question
Favorite

The collagen content in the liver was measured using the hydroxyproline content as described in [23]. The absorbance values at 558 nm were converted into µg units using the 4-parameter standard curve generated using the standards and expressed as µg hydroxyproline/g of tissue.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A