4.8. CBC Resistance Analyses

CBC resistance was assessed in plants after transient VIGS overexpression experiments, as reported previously [58]. Briefly, healthy mature leaves of each plant were selected and then punctured (0.5 mm) at six different places; then, each punctured site was injected with 1 µL of XccYN1 (1 × 10⁸ CFU∙mL⁻¹). Three sets of experiments were repeated. In the transiently overexpression experiments, three leaves were measured in each set, and in VIGS experiments, seven leaves were measured in each set. Leaves were then evaluated for CBC symptoms at 10 days post-inoculation (dpi), with the degree of CBC resistance assessed based on disease lesion area and disease index values [58].