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AsPC-1 and HPAF-II cells were harvested, diluted in RPMI1640 medium containing 10% FBS, and dispensed in 384-well plates at a concentration of 800 cells per well in 25 μL medium. After overnight incubation of the plates, 25 μL fresh medium with samples was added to each well and the plates were further incubated for 5 days. After the 5-day culture, 25 μL of CellTiter-Glo 2.0 reagent (Promega) was added to each well and the luminescence was quantified with an Envision microplate reader (PerkinElmer).
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