0.5–2 × 106 cells have been washed and resuspended in 500 µl of motility medium in 96well plate for 4 h. Then, cells have been collected and lactate has been measured by using Lactate Assay Kit (Sigma MAK064), following manufacturer instructions. Measurements were performed using CLARIOstar instrument (BMG Labtech, software version 5.6.1).
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