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Preparing the culture medium of mitis salivarius agar

HA Haya Doura Alomari
YT Yasser Alsayed Tolibah
CK Chaza Kouchaji
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The Petri dishes that contained the selective medium were previously prepared during the samples collecting period, by dissolving 90 g/L of Mitis Salivarius Agar (M-S Agar) (HiMedia, Maharashtra, India) in distilled water and adding 20% sucrose, then the mixture was placed in a wet sterilizer (JSAT-85; JSR®, Chungchungnam-Do, South Korea) at a temperature of 110 °C and a pressure of 15 psi for 15 min. When the mixture was cooled to 50 °C, the suspension of 0.2 IU/ml bacitracin (Fagron, Glinde, Germany) was added, then the medium was poured into sterile Petri dishes and they were left to cool. Afterward, they were put in the refrigerator upside down to prevent water condensation on the surface of the medium. The previous method of preparing the M-S Agar was similar to the method described by Tanzer [18].

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