Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Cryopreserved heart slices were fixed in 4% formaldehyde for 30 min at room temperature (RT), followed by three PBS washes and a 15-min HBSS priming period. Afterward, the cardiac slices were stained with WGA (Thermo Fisher Scientific; W11261) and left in the dark for 10 min at room temperature, followed by PBS washes and DAPI counterstaining. The cardiomyocyte surface area was measured using a fluorescence microscope at X60 magnification and computed using ImageJ (1.52a version; National Institute of Health USA).
Copyright and License information: The Author(s) ©2024
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
