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Flow cytometry

FY Fan Yang
MA Md Naushad Akhtar
DZ Duo Zhang
RE Rakan El-Mayta
JS Junyoung Shin
JD Jay F. Dorsey
LZ Lin Zhang
XX Xiaowei Xu
WG Wei Guo
SB Stephen J. Bagley
SF Serge Y Fuchs
CK Constantinos Koumenis
JL Justin D. Lathia
MM Michael J. Mitchell
YG Yanqing Gong
YF Yi Fan
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Single-cell suspensions derived from mouse tumors or human Mφs were stained with fluorescence dye-conjugated antibodies against CD11b (1:100; BioLegend 101206 or 101211), CD206 (1:100; BioLegend, 321110, 141707, or BD Biosciences, 551135), IL-10 (1:50; BD, 566568), F4/80 (1:100; Miltenyi Biotec, 130-102-327), IFN-γ (1:50; BioLegend, 502505), IL-10 (1:50; BioLegend, 505009), arginase 1 (1:50; eBioscience, 17-3697-80), or control immunoglobulin G (IgG). Cells were analyzed by using Accuri C6 (BD Biosciences) and FACSCanto II flow cytometers (BD Biosciences). The data were analyzed by FlowJo software (V10).

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