THP-1 cells were exposed to 10 or 12.5 μg/ml ZnO NPs. After 18 h exposure, the RNA was isolated using TOOLSharp RNA Extractor (TTD-NRNA200, BIOTOOLS Co., Ltd., Taipei, Taiwan). RNA concentrations and 260/280 nm ratios were measured by NanoDrop 2000 (Thermo Scientific™, Waltham, MA, USA). Reverse transcription (RT) was performed using TOOLSQuant II Fast RT Kit (KRT-BA06-2, BIOTOOLS Co., Ltd., Taipei, Taiwan). Finally, a mixture of the synthesized cDNA, primers and TOOLS 2 × SYBR qPCR Mix (FPT-BB05, BIOTOOLS Co., Ltd., Taipei, Taiwan) was carried out in a StepOnePlus™ Real-Time PCR System (Applied Biosystems, Foster, CA, USA) using SYBR Green Master Mix (Thermo Fisher Scientific). The average of the ΔΔC(t) of triplicate samples was calculated using β-actin as a housekeeping gene. The sequences of forward primers and reverse primers were summarized as described under Supplementary information.
Copyright and License information: The Author(s) ©2024 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.