4.11. Morphometric Analysis

Using pancreatic tissue slides stained with insulin-conjugated IHC antibodies, insulin-positive cells were detected. The overall number of islets and the number of islets exhibiting a positive response to insulin in 1 mm² of the pancreatic parenchyma (N/mm²) were also recorded. The percentage of insulin-positive cells in each individual islet was calculated by dividing the total number of islet cells by the number of cells that displayed positive insulin staining (Figure S3). The insulin content in β–cells was evaluated by measuring the intensity of insulin antigen expression in the islets according to their optical density, using the image analysis program Video Test “Morphology” 5.0 (VideoTesT, St. Petersburg, Russia).